大鼠组胺 elisa

小鼠组胺 elisa

人 组胺 elisa

其他种属 组胺 elisa

LDN ，Histamine（组胺）ELISA Fast Track  ，货号BA E-1700， 可以检测人，大鼠，小鼠，各种种属样本中的组胺含量。使用简便，结果准确，是全世界检测组胺的第一品牌，性价比高！并为多个一线ELISA品牌提供OEM 产品。

参数比较：

BioTNT （上海冠泰生物 www.biotnt.com）作为LDN的中国区代理, LDN 所有组胺产品如下：

•   Plasma, Urine and Whole Blood    Histamine  ELISA   BA E-1000

•   Histamine Release                                      BA E-1100

•   Cell Culture, Urine and Whole Blood   Histamine ELISA  Fast Track  BA E-1700

•   Any Biological Fluid/Sample              Histamine Research ELISA BA E-5800

•   Food Safety

ü  HistaSure™ ELISA High Sensitive         FC E-3100   

ü  HistaSure™ ELISA Fast Track (48 determinations)  FC E-3600

ü  HistaSure™ XL ELISA Fast Track (480 determinations)   FC E-3900

ü  HistaSure™ Fish Rapid Test    FC L-3200

Histamine ELISA  Fast Track说明书

货号： BA E-1700

1.      试剂盒用途和原理：

酶免疫法测定血浆和尿液中的含量

试剂盒可用于尿液和细胞上清中，以及全血中的组胺（Histamine）的定量检测。试剂盒可用于大鼠组胺（Histamine），小鼠组胺（Histamine）和人组胺（Histamine），以及其他动物的组胺（Histamine）的检测。组胺没有种属特异性，是否能用于检测主要和样本类型有关，以及与样本中的含量有关。

在程序的第一部分，组胺被定量酰化。 随后的竞争性ELISA试剂盒使用微孔板格式。 抗原与微孔板的固相结合，酰化标准品、对照品和样品以及固相结合分析物竞争固定数目的抗血清结合位点。 在体系平衡后，通过洗涤除去游离抗原和游离抗原-抗血清复合物。与固相结合的抗体被检测。以TMB为底物的抗兔IgG-过氧化物酶偶联物。 在450nm处监测反应。

通过已知的浓度的标准品制定标准曲线，未知样品的定量是通过将其吸光度与标准曲线回归进行计算。

2.   实验建议

2.1  测试结果的可靠性

为了确保对测试结果进行可靠的评估，必须根据所包含的说明以及当前的规则和指南(GLP, RILIBÄK等)进行评估。

在测试过程中，必须特别注意control 的精度和正确性的结果；这些control的结果必须在标准范围内。如果提供的质控报告（pre-set assay）与实际结果之间存在显著差异，请联系试剂盒制造商以获得进一步建议。

建议每个实验室建立自己的参考区间。本测试说明中报告的值仅用于参考。

本试剂盒获得的结果不应被视为任何治疗结果的唯一原因，而必须与其他诊断试验和临床观察相关联。

2.2   投诉

2.3   质量保证

该试剂盒根据最新技术生产，并经过严格的内部和外部质量控制检查来进行保证。检测试剂盒或检测操作步骤的任何改变以及不同批号试剂的混用可能会对检测结果产生负面影响，因此不在质保范围内。制造商对运输过程中发生的损坏不承担责任。

2.4   试剂盒残余物的处理

2.5   干扰

不要混合不同批次的试剂和溶液。考虑不同的运输和储存条件，测试样品处理不当，或实验操作不恰当都会影响结果。不要使用过期试剂盒。避免试剂和洗涤水受到微生物污染。温育和洗涤对实验会有影响。

2.6   预防措施

注意温育和洗涤说明。切勿用嘴吸移，避免试剂和样本与皮肤接触。在处理样品或试剂盒的区域，禁止吸烟、饮食。使用试剂盒或样品时，请务必戴上防护手套，完成工作后立即彻底洗手。避免任何形式的喷洒。避免皮肤接触试剂。使用防护服和一次性手套。所有步骤都必须根据protocol执行。只有使用校准过的移液器，才能获得最佳测试结果。叠氮化钠可能与铅和铜管发生反应，并可能形成高度爆炸性的金属叠氮化物。清理时，用大量水彻底冲洗，以防止这种形成。

3.   保存条件和稳定性

储存2-8℃。请勿使用超出标签上标明的过期日的试剂盒组分。

4.   试剂盒

4.1  试剂盒成分

4.2 需要但试剂盒中没有提供的材料和设备

- 可调微量移液器 (e.g. 10-100 µL / 100-1000µL)

- 洗板机

- 可 450 nm 和 620 或 650 nm读数的酶标仪

- 可吸水物 (纸塔)

- 双蒸水

- 混匀器

试剂盒实验时可以振荡温育或者不振荡。如果要振荡，请按照以下参数：shaking amplitude 3mm; approx. 600 rpm.

检测全血中的组胺，需要以下试剂（需另购）：

BA E-1726 Release Buffer  1 x 250 mL ready for use

5.   样本采集和保存

样本避免反复冻融

细胞上清：在2-8℃下最多6小时；在-20℃或者-80℃下长期(最长6个月)。

储存:在2-8℃下最多6小时；在-20℃下长期(最长6个月)。

尿液：可以使用收集在含有10-15毫升6 M盐酸的瓶中的自发性尿液或24小时尿液。储存:在-20℃下储存更长时间(最长6个月)。避免直接暴露在阳光下。

全血：组胺的释放是用肝素化的全血进行的。

保存：20-25度；远离阳光和直接的热源；

稳定性：24小时。不能冷藏，白细胞会凝结。

6.   实验protocol

让所有试剂和样品达到室温。建议进行双重复测试。

6.1 准备试剂

Wash Buffer洗涤缓冲液

用蒸馏水将20毫升Wash Buffer Concentrate稀释至最终体积为1000毫升。

储存:最高6个月2–8℃；

酰化试剂Acylation Solution：

Acylation Solution具有18.5℃的冰点。为了确保Acylation Solution在使用时是液体，必须确保已经达到室温，并且在使用前形成均匀、无晶体的溶液。替代地，酰化稀释剂可以储存在室温(20–25℃)下，与其他试剂盒组分分开。

全血

为了测定全血中总组胺，用Release Buffer (BA E-1726)稀释肝素化全血1:20，并在90℃孵育10分钟(例如50ul全血加1毫升释放缓冲液。)

在2–8℃孵育10分钟，让样品冷却。

在700 x g下离心10分钟(制动器关闭)

6.2

1. 样品制备和酰化： 吸移管100µL 的standards ，control 以及血浆样品 ，20µL 的细胞上清液或尿样， 或100µL 的 预处理的全血样本（见6.1）加入reaction plate的孔中；

2.  细胞上清液或尿液孔中加入80µL 的Diluent （补足100ul）。 

3. 加入25µL 的酰化溶液（Acylation Solution）到所有孔中。

5.每孔加入200µL 的Acylation Buffer。 

6. 在振荡器上（approx. 600 rpm）温育15分钟， 室温 (20-25°C)。

取25µL制备品进入下一步ELISA 实验

6.3  histamine ELISA

Discard or aspirate the contents of the wells and wash each well 3 times thoroughly with 300 µL Wash
Buffer. Blot dry by tapping the inverted plate on absorbent material.

shaker（振荡器）的使用不是强制性的。提供没有shaker的替代方案；

1.   向Histamine Microtiter Strips的相应板条的孔中加入 25 µL 酰化好的标准品，controls 和样品；

2.   加入100 µL 的Histamine Anti serum 到各个孔中；封板；

3.   在振荡器上孵育30分钟(20-25℃)(大约600转/分)；

不使用振荡器: 用手轻轻摇动旋转微孔板，并在室温(2-8℃)下孵育40分钟；

4.   撕掉膜，倒掉孔里的液体，用洗液洗板洗三次，每次300ul 洗液每孔。在纸塔上拍干；

5.   加入100 µL的Enzyme Conjugate到每孔中；

6.   在振荡器上孵育10分钟(20-25℃)(大约600转/分)；

7.   倒掉孔里的液体，用洗液洗板洗三次，每次300ul 洗液每孔。在纸塔上拍干；

8.   加入100 µL的底物液到每孔中；

9.   在振荡器上孵育15 ± 2 min (20-25℃)(大约600转/分)；避光；

10.           加入100 µL的Stop solution 到每孔中；振荡微孔板，保证溶液的均匀； 

11.           在10分钟内，使用酶标仪450nm读数，可设置参比波长为620nm 或650nm；

7.   计算结果；

校准曲线是通过将标准品（线性，Y轴）的吸光度读数（计算平均吸光度）与相应的标准浓度（对数，X轴）作图而获得的。

使用非线性回归进行曲线拟合（例如，样条，4参数，Akima）。

质控可直接从校准曲线读取。 

尿液中的组胺读数必须乘以5； 

24h尿中组胺排泄总量计算如下:   µg/24h=µg/L x L/24h 

细胞上清中的组胺读数必须乘以5；

全血样本中的组胺读数必须乘以21；

在溢出的情况下，使用设置为405nm的酶标仪读数，在10分钟内读取孔中溶液的吸光度。

7.3典型校准曲线（仅供参考）

预期值：

更多信息见英文说明书：http://www.biotnt.com/product/product_134032.html

8.  组胺ELISA 试剂盒发表文章列表：

1)     AUTHOR: Clubb et al ， TITLE: Comparison of Selected Diagnostic Parameters in African Grey Parrots (Psittacus erithacus) with Normal Plumage and Those Exhibiting Feather Damaging Behavior ，YEAR: 2007   ANALYTE: Histamine ；  METHODE: ELISA 

JOURNAL: Journal of Avian Medicine and Surgery | NUMBER: 21 (4) | PAGES: 259-264

2)     AUTHOR: Reil et al.        TITLE: Insights from knock-out models concerning postischemic release of TNFalpha from isolated mouse hearts        YEAR: 2007      

ANALYTE: Histamine   METHODE: ELISA       

JOURNAL: Journal of Molecular and Cellular Cardiology | NUMBER: 42(1) | PAGES: 133-141

3)         AUTHOR: Rudolph et al.           TITLE: The Influence of Mast Cell Mediators on Migration of SW756 Cervical Carcinoma Cells       YEAR: 2008         

ANALYTE: Histamine    METHODE: ELISA           

JOURNAL: Journal of Pharmacology Sciences | NUMBER: 106 | PAGES: 208-218

4)     AUTHOR: Amagai et al.         TITLE: Establishment of a novel high-affinity IgE receptor-positive canine mast cell line with wild-type c-kit receptors      YEAR: 2008      

ANALYTE: Histamine cell culture    METHODE: ELISA  

JOURNAL: Biochemical and Biophysical Research Communications | NUMBER: 366(3) | PAGES: 857-8615）  

5)      AUTHOR: Maintz et al.         TITLE: Contribution of histamine metabolism to tachyphylaxis during the buildup phase of rush immunotherapy       YEAR: 2009      

ANALYTE: Histamine       METHODE: ELISA        

6)     AUTHOR: Yang et al.            TITLE: Immunomodulatory Effects of Egg White Enzymatic Hydrolysates Containing Immunodominant Epitopes in a BALB/c Mouse Model of Egg Allergy      

YEAR: 2009  

ANALYTE: Histamine      METHODE: ELISA  

JOURNAL: Journal of Agricultural and Food Chemistry | NUMBER: 57(6) | PAGES: 2241-2248

7)       AUTHOR: Grant et al.         TITLE: Effects of Gadolinium Contrast Agents in Naïve and Nephrectomized Rats: Relevance to Nephrogenic Systemic Fibrosis YEAR: 2009  

ANALYTE: Histamine                                                                                                                 METHODE: ELISA        

JOURNAL: Acta Radiologica | NUMBER: 50(29 | PAGES: 156-169

8)     AUTHOR: Kemp et al.                  TITLE: The relationship between the rapid rejection of Haemonchus contortus larvae with cells and mediators in abomasal tissues in immune sheep      

YEAR: 2009  

ANALYTE: Histamine  METHODE: ELISA         

JOURNAL: International Journal for Parasitology | NUMBER: 39 | PAGES: 1589-159410）

9)     AUTHOR: Passante et al.       TITLE: RBL-2H3 cells are an imprecise model for mast cell mediator release       YEAR: 2009      

ANALYTE: Histamine                                                                                                                 METHODE: ELISA        

JOURNAL: Inflammation Research | NUMBER: 58(9) | PAGES: 611-618                                                                                                                                                                                         

10)    AUTHOR: Kemp et al.                TITLE: The relationship between the rapid rejection of Haemonchus contortus larvae with cells and mediators in abomasal tissues in immune sheep     

YEAR: 2009        

ANALYTE: Histamine Cell Culture    METHODE: ELISA       

JOURNAL: International Journal for Parasitology

11)   AUTHOR: Yang et al.    TITLE: Multiple T cell epitope peptides suppress allergic responses in an egg allergy mouse model by the elicitation of forkhead  box transcription factor 3- and transforming growth factor-?-associated mechanisms        YEAR: 2010      

ANALYTE: Histamine  METHODE: ELISA         

JOURNAL: Clinical & Experimental Allergy | NUMBER: 40 (4) | PAGES: 668-67

12)   AUTHOR: Kidd et al.       TITLE: The CCK2 receptor antagonist, YF476, inhibits Mastomys ECL cell hyperplasia and gastric carcinoid tumor development YEAR: 2010     

ANALYTE: Histamine      METHODE: ELISA         

JOURNAL: Regulatory Peptides | NUMBER: 162 (1-3) | PAGES: 52-60

13)   AUTHOR: Rozenberg et al.      TITLE: Histamine H1 Receptor Promotes Atherosclerotic Lesion Formation by Increasing Vascular Permeability for Low-Density

Lipoproteins    YEAR: 2010      

ANALYTE: Histamine   METHODE: ELISA       

JOURNAL: Arteriosclerosis, thrombosis, and vascular biology | NUMBER: 30 | PAGES: 923-930

14)    AUTHOR: Rozenberg et al.                                                                                                                                         TITLE: Histamine H1 Receptor Promotes Atherosclerotic Lesion Formation by Increasing Vascular Permeability for Low-Density

Lipoproteins                                                                                                                                                                     YEAR: 2010                                                                                                                                                                                             

ANALYTE: Histamine      METHODE: ELISA                                                                                                    

JOURNAL: Arteriosclerosis, thrombosis, and vascular biology | NUMBER: 30 | PAGES: 923-930

15)    AUTHOR: Rozenberg et al.                                                                                                                                         TITLE: Histamine H1 Receptor Promotes Atherosclerotic Lesion Formation by Increasing Vascular Permeability for Low-Density Lipoproteins                                                                                                                                                                     YEAR: 2010                                                                                                                                                                                             

ANALYTE: Histamine      METHODE: ELISA                                                                                                    

JOURNAL: Arteriosclerosis, thrombosis, and vascular biology | NUMBER: 30 | PAGES: 923-930

16)    AUTHOR: Toba et al.                                                                                                                                                   TITLE: Fibroblast-specific expression of AC6 enhances ß-adrenergic and prostacyclin signaling and blunts bleomycin-induced

pulmonary fibrosis                                                                                                                                                         YEAR: 2010                                                                                                                                                                                             

17)    ANALYTE: Histamine    METHODE: ELISA                                                                                                        

JOURNAL: Clinical and Experimental Pharmacology and Physiology | NUMBER: 37 | PAGES: 62-68

AUTHOR: Weaver et al                                                                                                                                                 TITLE: Early Events in Vascular Injury in the Rat Induced by the Phosphodiesterase IV Inhibitor SCH 351591   YEAR: 2010       

ANALYTE: Histamine / Serotonin       METHODE: ELISA                                                                             

JOURNAL: Toxicology Pathology | NUMBER: 38 | PAGES: 738-744

18)    AUTHOR: Toba et al.                                                                                                                                                     TITLE: Oral L-histidine exerts antihypertensive effects via central histamine H? receptors and decreases nitric oxide content in the rostral ventrolateral medulla in spontaneously hypertensive rats                                                                                    

YEAR: 2010                                                                                                                                                                 

ANALYTE: Histamine                                                                                                                                                 

JOURNAL: Clinical and Experimental Pharmacology and Physiology | NUMBER: 37 | PAGES: 62-68

19)    AUTHOR: Toba et al.                                                                                                                                                     TITLE: Oral L-histidine exerts antihypertensive effects via central histamine H? receptors and decreases nitric oxide content in the rostral ventrolateral medulla in spontaneously hypertensive rats                                                                                    

YEAR: 2010                                                                                                                                                                 

ANALYTE: Histamine                                                                                                                                                 

JOURNAL: Clinical and Experimental Pharmacology and Physiology | NUMBER: 37 | PAGES: 62-68

20)    AUTHOR: Garcia-Faroldi et al.                                                                                                                                TITLE: Polyamines Are Present in Mast Cell Secretory Granules and Are Important for Granule HomeostasisYEAR: 2010  

ANALYTE: Histamine/Serotonin     METHODE: ELISA                                                                                   

JOURNAL: PLoS One | NUMBER: 5(11)

21)    AUTHOR: Chirumbolo et al.                                                                                                                                        TITLE: Bimodal action of the flavonoid quercetin on basophil function: an investigation of the putative biochemical targets YEAR: 2010                                                                                                                                                                              

ANALYTE: Histamine      METHODE: ELISA                                                                                                    

JOURNAL: Clinical and Molecular Allergy (CMA) | NUMBER: 8(13)

22)    AUTHOR: Lopez-Font et al.                                                                                                                                         TITLE: Pancreatic and pulmonary mast cells activation during experimental acute pancreatitis                                 YEAR: 2010                                                                                                                                                                                             

ANALYTE: Histamine         METHODE: ELISA                                                                                              

JOURNAL: World Journal of Gastroenterology (WJG) | NUMBER: 16(27) | PAGES: 3411-3417

23)    AUTHOR: Woska et al.                                                                                                                                                TITLE: Small-Interfering RNA-Mediated Identification and Regulation of the Ternary SNARE Comlpex Mediating RBL-2H3 Mast CellDegranulation                                                                                                                                                           YEAR: 2010                                                                                                                                                                                             

ANALYTE: Histamine  METHODE: ELISA                                                                                                            

JOURNAL: Basic Immunology | NUMBER: 73(1)

24)    AUTHOR: Rupa et al.                                                                                                                                                     TITLE: Oral immunotherapy with immunodominant T-cell epitope peptides alleviates allergic reactions in a Balb/c mouse model of egg allergy                                                                                                                                                                            

YEAR: 2011                                                                                                                                                                 

ANALYTE: Histamine     METHODE: ELISA                                                                                                      

JOURNAL: Allergy

25)    AUTHOR: Osterday et al.                                                                                                                                             TITLE: Vitamin C and Treating the Common Cold                                                                                                               YEAR: 2011                                                                                                                                                                                             

ANALYTE: Histamine    METHODE: ELISA                                                                                                        

JOURNAL: Dissertation

26)    AUTHOR: Köse et al.                                                                                                                                                   TITLE: Commercial test kits and the determination of histamine in traditional (ethnic) fish products-evaluation against an EU accepted HPLC method                                                                                                                                               YEAR: 2011                                                                                                                                                                                             

ANALYTE: Histamine     METHODE: ELISA                                                                                                      

JOURNAL: Food Chemistry | NUMBER: 125 | PAGES: 1490-1497

27)    AUTHOR: Köse et al.                                                                                                                                                   TITLE: Commercial test kits and the determination of histamine in traditional (ethnic) fish products-evaluation against an EU  accepted HPLC method                                                                                                                                               YEAR: 2011                                                                                                                                                                                             

ANALYTE: Histamine    METHODE: ELISA                                                                                                        

JOURNAL: Food Chemistry | NUMBER: 125 | PAGES: 1490-1497

28)    AUTHOR: Jiménez-Saiz et al.                                                                                                                                     TITLE: Immunomodulatory Effects of Heated Ovomucoid-Depleted Egg White in a BALB/c Mouse Model of Egg Allergy        YEAR: 2011                                                                                                                                                                 

ANALYTE: Histamine         METHODE: ELISA                                                                                              

JOURNAL: Journal of Agricultural and Food Chemistry | NUMBER: 59(24) | PAGES: 13195-13202

29)   AUTHOR: Gourbeyre et al.           TITLE: Wheat gliadins modified by deamidation are more efficient than native gliadins in inducing a Th2 response in Balb/c mice

exp erimentally sensitized to wheat allergens          YEAR: 2011      

ANALYTE: Histamine 

METHODE: ELISA        

JOURNAL: Molecular Nutrition & Food Research

30)   AUTHOR: Koo et al.        TITLE: Cochlear uptake of fluorescent gentamicin is increased in LPS-induced sepsis model          YEAR: 2011      

ANALYTE: Histamine / Serotonin

METHODE: ELISA        

JOURNAL: Dissertation

31)   AUTHOR: Husain et al.          TITLE: Mesenteric lymph node transcriptome profiles in BALB/c mice sensitized to three common food        YEAR: 2011      

ANALYTE: Histamine   METHODE: ELISA       

JOURNAL: BMC Genomics | NUMBER: 12(12)

32)   AUTHOR: Davis et al.            TITLE: Up-regulation of histidine decarboxylase expression and histamine content in B16F10 murine melanoma cells        YEAR: 2011      

ANALYTE: Histamine                                                                                                                METHODE: ELISA        

JOURNAL: Inflammation Research | NUMBER: 60(1) | PAGES: 55-61

33)   AUTHOR: Davis et al.            TITLE: Up-regulation of histidine decarboxylase expression and histamine content in B16F10 murine melanoma cells        YEAR: 2011      

ANALYTE: Histamine  METHODE: ELISA         

JOURNAL: Inflammation Research | NUMBER: 60(1) | PAGES: 55-61

34)   AUTHOR: Sang-Chul et al.           TITLE: Fermented fish oil supresses T helper 1/2 cell response in a mouse model of atopic dermatitis via generation of CD4 +

CD25 + Foxp3 + T cells        YEAR: 2012      

ANALYTE: Histamine  METHODE: ELISA         

JOURNAL: BMC Immunology | NUMBER: 13(44)

35)   AUTHOR: Yang et al.    TITLE: Prophylaxis of Intranasally Induced Pollen Allergy in a BALB/C Mouse Model Using a Potential Prebiotic β-1,4 Mannobiose YEAR: 2012    

ANALYTE: Histamine  METHODE: ELISA         

JOURNAL: Allergology International

36)   AUTHOR: Martin et al.          TITLE: Influence of Dietary Histidine on Basophil Release, Circulating Concentration, and Urinary Excretion of Histamine in Dome-stic Felines      YEAR: 2012      

ANALYTE: Histamine  METHODE: ELISA         

JOURNAL: Intern J Appl Res Vet Med. | NUMBER: 10(4) | PAGES: 289-299

37)   AUTHOR: Hox et al.        TITLE: Crucial role of TRPA1 and mast cells in induction of non-allergic airway hyperreactivity in mice         YEAR: 2012      

ANALYTE: Histamine   METHODE: ELISA       

JOURNAL: American Journal of Respiratory and Critical Care Medicine

38)   AUTHOR: Boskabady et al.          TITLE: The effect of the extract of Crocus sativus and its constituent safranal, on lung pathology and lung inflammation

of ovalbumin sensitized guinea-pigs          YEAR: 2012      

ANALYTE: Histamine    METHODE: ELISA      

JOURNAL: Phytomedicine | NUMBER: 19 | PAGES: 904-911

39)   AUTHOR: Bertl et al.     TITLE: Smoking influences salivary histamine levels in periodontal disease          YEAR: 2012  

ANALYTE: Histamine  METHODE: ELISA         

JOURNAL: Oral Diseases | NUMBER: 18(4) | PAGES: 410-416

40)   AUTHOR: Bertl et al.     TITLE: Smoking influences salivary histamine levels in periodontal disease          YEAR: 2012  

ANALYTE: Histamine      METHODE: ELISA  

JOURNAL: Oral Diseases | NUMBER: 18(4) | PAGES: 410-416

41)   AUTHOR: Yagci et al.     TITLE: TCTP/HRF pathway and angiogenesis: A feasible intercourse in chronic lymphocytic leukemia           YEAR: 2013      

ANALYTE: Histamine     METHODE: ELISA    

JOURNAL: Leukemia Research | NUMBER: 37 | PAGES: 665-670

9.    LDN 公司和其他产品介绍：

德国LDN，全名labor diagnostika nord，是生物胺、神经递质检测的行业领先公司。 本试剂盒可用于人/大鼠/小鼠/各种动物样本，血清/抗凝血浆样本。

LABOR DIAGNOSTIKA NORD在德国始建于1996年， 目的是向临床和科研领域产品提供优质的检测产品和系统。最早的生物胺检测ELISA试剂，是LDN的ELISA系列核心，目前是全世界最大的生物胺检测试剂提供商；推荐特色产品是儿茶酚胺（catecholamines）检测系列，Histamine（组胺）检测ELISA，组胺（血清素）ELISA 试剂盒，自由Metanephrine（3-甲氧基肾上腺素）在血浆中的检测ELISA ；

 随后发展的内分泌检测系列和食物安全系列为德国LDN 另外两大产品系列。

LABOR DIAGNOSTIKA NORD  产品在全世界八十多个国家通过代理商或者OEM 合作伙伴进行销售，在北美洲通过子公司Rocky Mountain Diagnostics（ROCKY mountain诊断公司）进行销售。

 优势产品有：Histamine（组胺） ELISA ，Noradrenaline（去甲肾上腺素） ELISA,  Dopamine（多巴胺）ELISA, Serotonin ( 血清素，又名5羟色胺）ELISA等。根据不同的样本类型，分为Fast Track （快速），research 型，高敏型等多种类型；既有针对所有类型样本的research类型的，也有针对血清，血浆或者尿液的试剂盒；这些试剂盒都可用于所有animal的检测（如人，小鼠，大鼠，其他动物模型等）。

具体见下表：

 德国LDN生物胺，神经递质ELSIA 试剂盒:

指标主要有：儿茶酚胺（catecholamines）检测试剂盒：Adrenaline（肾上腺素），Dopamine （多巴胺），Noradrenaline（去甲肾上腺素）；

以及组合检测试剂盒：2-CAT（A-N）， 2-CAT（N-D），3-CAT；

Histamine（组胺），有针对尿液，血清，全血检测的试剂盒，也有针对细胞上清/尿液/全血检测的试剂盒；还有针对所有样本检测的试剂盒；检测粪便的组胺试剂盒；

Metanephrine（3-甲氧基肾上腺素），Normetanephrine（去甲-3-0-甲基肾上腺素），Serotonin（5-HT，色氨酸，5羟色胺），5-HIAA（5-吲哚乙酸），Glutamate（谷氨酸），GABA（氨基丁酸），Tryptophan（色氨酸）；

既有针对所有类型样本的research类型的，也有针对血清，血浆或者尿液的试剂盒；

这些试剂盒都可用于所有animal的检测（如人，小鼠，大鼠，牛，羊，猪，豚鼠，猴，兔等其他动物模型）。