大鼠5-羟色胺 elisa
小鼠5-羟色胺 elisa
人 5-羟色胺 elisa
其他种属 5-羟色胺 elisa
LDN ，Serotonin ELISA（5-羟色胺）Fast Track ，货号BA E-8900， 可以检测人，大鼠，小鼠，各种种属样本中的血清素（5-HT, 5-羟色胺）含量。使用简便，结果准确，是全世界检测serotonin的第一品牌，性价比高！并为多个一线ELISA品牌提供OEM 产品。
BioTNT （上海冠泰生物 www.biotnt.com）作为LDN的中国区代理，这款热销产品Serotonin ELISA（5-羟色胺）Fast Track BA E-8900备现货。
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货号:
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BA E-8900
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品名
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Serotonin ELISA Fast Track
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描述
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快速ELISA 定量检测5-羟色胺（血清素）
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设计
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即用型设计， Acylation in liquid phase. 6 standards, 2 controls
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样本用量
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25 µl serum, urine, platelets（血清，尿液，血小板）
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整体实验时间
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乙酰化15 分钟，ELISA 1 h
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标准曲线范围
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0 / 15 - 2 500 ng/ml
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灵敏度
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6.2 ng/ml
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保存条件
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2 - 8 °C
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试剂盒规格
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96孔
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试剂盒优点:
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· 整体反应时间. 1h 15 min
· 已知药物对反应无影响
· 不需要稀释，定量范围宽；
· 与其他的方法学相比，关联性好（HPLC）
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CE MARK:
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是的，通过欧洲CE 认证。
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另外，Serotonin Research ELISA（高敏)（5-羟色胺）（人/大鼠/小鼠/各种属通用） BA E-5900可以检测更多类型的样本。
Serotonin ELISA （fast track） 说明书
货号： BA E-8900
1. 试剂盒用途和原理：
试剂盒可用于血清、尿液和血小板样本中血清素（5-HT, 5-羟色胺）的定量检测。试剂盒可用于大鼠血清素（5-HT, 5-羟色胺），小鼠血清素（5-HT, 5-羟色胺）和人血清素（5-HT, 5-羟色胺），以及其他动物的血清素（5-HT, 5-羟色胺）的检测。
第一步，血清素被定量酰化。随后, 竞争性酶联免疫试剂盒使用微孔板形式。抗原结合在微孔板上，酰化的标准品、对照品和样品以及固相结合的分析物竞争固定数量的抗血清结合位点。系统平衡后，通过洗涤除去游离抗原和游离抗原-抗血清复合物。使用TMB作为底物，通过anti-兔IgG-过氧化物酶复合物检测与固相结合的抗体。酶标仪450nm 读数。
通过已知的浓度的标准品制定标准曲线，未知样品的定量是通过将其吸光度与标准曲线回归进行计算。
2. 实验建议
2.1 测试结果的可靠性
为了确保对测试结果进行可靠的评估，必须根据所包含的说明以及当前的规则和指南(GLP, RILIBÄK等)进行评估。
在测试过程中，必须特别注意control 的精度和正确性的结果；这些control的结果必须在标准范围内。如果提供的质控报告（pre-set assay）与实际结果之间存在显著差异，请联系试剂盒制造商以获得进一步建议。
建议每个实验室建立自己的参考区间。本测试说明中报告的值仅用于参考。
本试剂盒获得的结果不应被视为任何治疗结果的唯一原因，而必须与其他诊断试验和临床观察相关联。
2.2 投诉
如有投诉，请向制造商提交一份书面报告，其中包含关于如何进行测试的所有数据、收到的结果以及原始测试打印件的副本。请联系制造商获取表格，并将其完整填写后返还给制造商。
2.3 质量保证
该试剂盒根据最新技术生产，并经过严格的内部和外部质量控制检查来进行保证。检测试剂盒或检测操作步骤的任何改变以及不同批号试剂的混用可能会对检测结果产生负面影响，因此不在质保范围内。制造商对运输过程中发生的损坏不承担责任。
2.4 试剂盒残余物的处理
2.5 干扰
不要混合不同批次的试剂和溶液。考虑不同的运输和储存条件，测试样品处理不当，或实验操作不恰当都会影响结果。不要使用过期试剂盒。避免试剂和洗涤水受到微生物污染。温育和洗涤对实验会有影响。
2.6 预防措施
注意温育和洗涤说明。切勿用嘴吸移，避免试剂和样本与皮肤接触。在处理样品或试剂盒的区域，禁止吸烟、饮食。使用试剂盒或样品时，请务必戴上防护手套，完成工作后立即彻底洗手。避免任何形式的喷洒。避免皮肤接触试剂。使用防护服和一次性手套。所有步骤都必须根据protocol执行。只有使用校准过的移液器，才能获得最佳测试结果。叠氮化钠可能与铅和铜管发生反应，并可能形成高度爆炸性的金属叠氮化物。清理时，用大量水彻底冲洗，以防止这种形成。
本检测试剂盒中包含人或动物血清或血浆的所有试剂均已通过FDA批准的程序检测并确认艾滋病毒I/II、乙型肝炎表面抗原和丙型肝炎病毒呈阴性
然而，所有试剂在使用和处置时都应被视为潜在的生物危害。
3. 保存条件和稳定性
将试剂(除了酰化试剂(BA E-8912))储存在2-8℃直至过期。酰化试剂应储存在室温下。请勿使用超出标签上标明的过期日的试剂盒组分。
4. 试剂盒
4.1 试剂盒成分
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BA D-0023
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反应管*）
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2 x 50
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准备使用
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BA E-0030
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Wash Buffer
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1 x 20毫升
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浓缩，用双蒸水稀释内容物，
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Concentrate
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最终体积为1000毫升
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BA E-0045
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Conjugate
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1 x 12毫升
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即用型，抗兔IgG偶联
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过氧化物酶
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BA E-0055
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substrate
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1 x 12毫升
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即用型，四甲基联苯胺(TMB)
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BA E-0080
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stop solution
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1 x 12毫升
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即用型，含0.25M 当量的硫酸
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BA E-0931
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serotonin-5-HIAA
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1x96wells
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12条，每条8孔，拆分，预包被
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Microtiter Strips
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BA R-8901
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标准A
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1 x 4毫升
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即用型
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BA R-8902
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标准B
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1 x 4毫升
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即用型
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BA R-8903
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标准C
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1 x 4毫升
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即用型
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BA R-8904
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标准D
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1 x 4毫升
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即用型
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BA R-8905
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标准E
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1 x 4毫升
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即用型
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BA R-8906
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标准F
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1 x 4毫升
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即用型
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BA E-8910
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Serotonin
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1 x 12毫升
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兔源，即用型，蓝色管，
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Antiserum
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蓝色螺帽
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BA E-8911
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Acylation Buffer
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1 x 55毫升
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即用型
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BA E-8912
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Acylation Reagent
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1 x 3毫升
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即用型
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BA R-8951
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Control 1
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1 x 4毫升
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即用型
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BA R-8952
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Control 2
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1 x 4毫升
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即用型
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*)也可以使用48孔板代替反应管进行样品制备和酰化(请参考6.2)。这些板可根据要求提供(货号BA D -0033)。
4.2 需要但试剂盒中没有提供的材料和设备
- 可调微量移液器 (e.g. 10-100 µL / 100-1000µL)
- 洗板机
- 可 450 nm 和 620 或 650 nm读数的酶标仪
- 可吸水物 (纸塔)
- 双蒸水
- 混匀器
试剂盒实验时可以振荡温育或者不振荡。如果要振荡，请按照以下参数：shaking amplitude 3mm; approx. 600 rpm.
5. 样本采集和保存
样本避免反复冻融
血清
溶血样品，尤其是血脂样品，不应用于本试验。
储存:在2-8℃下最多6小时；在-20℃下长期(最长6个月)。
尿
可以使用收集在含有10-15毫升6 M盐酸的瓶中的自发性尿液或24小时尿液。储存:在-20℃下储存更长时间(最长6个月)。避免直接暴露在阳光下。
血浆
超过98%的循环血清素位于血小板中，并在凝血过程中释放。血液必须通过静脉穿刺收集在含有乙二胺四乙酸(EDTA)或柠檬酸盐的塑料管中。
富含血小板的血浆(PRP)
为了获得富含血小板的血浆(PRP)，样品在室温(200g)下离心10分钟。将上清液转移到另一个试管中并计数血小板。
血小板
将800ul 生理盐水加入200ul PRP(包含350，000-500，000血小板/ul)并离心(4，500×g，4℃下10分钟)获得血小板颗粒。然后弃去上清液。
200ul双蒸水加入血小板中，在涡流混合器上充分混合。这种悬浮液可以在<-20℃冷冻储存几周。
冷冻样品解冻后，在室温下以10，000×g离心2分钟。
25 µL上清液用于酰化反应。
注意：对于血小板缺乏血浆和脑脊液中血清素的测定，应使用血清素研究酶联免疫吸附试验(BA E-5900)。
Test procedure for serum, urine and platelets
Allow all reagents and samples to reach room temperature. The measurement in duplicates is recommended.
6. 血清、尿液和血小板的测试程序
让所有试剂和样品达到室温。建议进行双重复测试。
6.1 准备试剂
Wash Buffer洗涤缓冲液
用蒸馏水将20毫升Wash Buffer Concentrate稀释至最终体积为1000毫升。
储存:最高6个月2–8℃；
Acylation Reagent酰化试剂
酰化试剂(BA E-8912)的冰点为18.5℃，为了确保酰化试剂在使用时是液体，必须确保酰化试剂达到室温，并在使用前形成均匀、无晶体的溶液。另外，酰化试剂可以在室温(20–25℃)下储存，与其他试剂盒组分分开(请参考3)。
6.2血清、尿液和血小板的样品制备和酰化
1. 25ul的standards，25ul 的controls，25ul的血清，尿液或血小板样品分别加入相应的反应管中。
2. 加入500ul的Acylation Buffer到每个管中；
3. 加入 25ul 的Acylation Reagent到每个管中；
4. 混合均匀，室温(20-25°C)温育15分钟(20-25°C)。
取25ul 血清素酶联免疫吸附试验的标准品、对照品和样品
6.3 Serotonin ELISA
shaker（振荡器）的使用不是强制性的。提供没有shaker的替代方案；
1. 向Serotonin Microtiter Strips的相应板条的孔中加入 25 µL 酰化好的标准品，controls 和样品；
2. 加入100 µL 的Serotonin Anti serum 到各个孔中；
3. 在振荡器上孵育30分钟(20-25℃)(大约600转/分)；
不使用振荡器: 用手轻轻摇动旋转微孔板，并在室温(20-25℃)下孵育1小时；
4. 倒掉孔里的液体，用洗液洗板洗三次，每次300ul 洗液每孔。在纸塔上拍干；
5. 加入100 µL的Conjugate到每孔中；
6. 在振荡器上孵育15分钟(20-25℃)(大约600转/分)；
不使用振荡器: 用手轻轻摇动旋转微孔板，并在室温(20-25℃)下孵育15分钟；
7. 倒掉孔里的液体，用洗液洗板洗三次，每次300ul 洗液每孔。在纸塔上拍干；
8. 加入100 µL的底物液到每孔中；
9. 在振荡器上孵育15 ± 2 min (20-25℃)(大约600转/分)；
不使用振荡器: 用手轻轻摇动旋转微孔板，并在室温(20-25℃)下孵育15 ± 2 min；避光；
10. 加入100 µL的Stop solution 到每孔中；振荡微孔板，保证溶液的均匀；
11. 在10分钟内，使用酶标仪450nm读数，设置参比波长为620nm 或650nm；
7. 计算结果；
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标准品浓度
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标准
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a
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b
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c
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d
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E
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F
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5-羟色胺（ng/ml）
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0
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15
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50
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150
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500
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2 500
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5-羟色胺（nmol/L）
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0
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85.1
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284
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851
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2 840
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14 175
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转换:
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5-羟色胺（ng/ml）x 5.67=5-羟色胺（nmol/L）
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校准曲线是通过将标准品（线性，Y轴）的吸光度读数（计算平均吸光度）与相应的标准浓度（对数，X轴）作图而获得的。
使用非线性回归进行曲线拟合（例如，样条，4参数，Akima）。
尿以及 血清样本可直接从校准曲线读取。
血小板中5-羟色胺的计算
血小板中5-羟色胺的含量参考109血小板。
说明性示例:
5-羟色胺测定浓度:100ng/ml
PRP中的血小板数:300 000/µL=0.3 x 109血小板/ml，5-羟色胺含量为100ng。
血小板中的5-羟色胺含量为:
333纳克/109血小板（100 ng血清素x 1.0 x 10 9/0.3 x 10 9）
7.1质量控制
建议根据州和联邦法规使用质控样品。 在正常和病理水平上使用对照。 试剂盒或其他商用质控品应在规定的置信限内。 试剂盒质控品的置信限打印在QC报告上。
7.2校准
抗血清与酶偶联物的结合和所用酶的活性是温度依赖性的，如果不使用恒温器，则OD值可能变化。 温度越高，OD值越高。 相应的变化也适用于孵育时间。 酶免疫分析的最佳温度为20-25°C。
在溢出的情况下，使用设置为405nm的酶标仪读取器，在10分钟内读取孔中溶液的吸光度
7.3典型校准曲线:
5-羟色胺（Serotonin）
2
1，8
1，6
1，4
1，2
1
0，8
0，6
0，4
0，2
0
10 100 1000 10000
ng/ml
示例。 请勿用于计算！
版本:10.0 生效日期:2011年9月1日 5/6
8. 更多信息见英文说明书：http://www.biotnt.com/file/BioTNTPDF/148@BA%20E-8900.pdf
9. 发表文章列表：
1) AUTHOR:Zhang et al.，TITLE:Bcl-2 family proteins are essential for platelet survival，YEAR:2007，ANALYTE:Serotonin，METHODE:ELISA，JOURNAL:Cell Death and Differentiation |NUMBER:14 |PAGES:943-951
2) AUTHOR: Kidd et al. TITLE: Further delineation of the continuous human neoplastic enterochromaffin cell line, KRJ-I, and the inhibitory effects of
a) lanreotide and rapamycin YEAR: 2007
b) ANALYTE: Serotonin METHODE: ELISA
c) JOURNAL: Journal of Molecular Endocrinology | NUMBER: 38 | PAGES: 181-192
3) AUTHOR: Briffa et al. TITLE: Monoamines and decision making during contests in the hermit crab Pagurus bernhardus YEAR: 2007
a) ANALYTE: Serotonin Research METHODE: ELISA
b) JOURNAL: Animal Behaviour | NUMBER: 73 | PAGES: 605-612
4) AUTHOR: Poon et al. TITLE: Bystander effects of ionizing r adiation can be modulated by signaling amines YEAR: 2007
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Enviromental Research | NUMBER: 105(2) | PAGES: 200-211
5) AUTHOR: Soga et al. TITLE: Serotonin Activates Human Monocytes and Prevents Apoptosis YEAR: 2007
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Journal of Investigative Dermatology | NUMBER: 127(8) | PAGES: 1947-1955
6) AUTHOR: Van Buren et al. TITLE: The Development and Characterization of a Human Midgut Carcinoid Cell Line YEAR: 2007
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Clinical Cancer Research | NUMBER: 13(16) | PAGES: 4704-4712
7) AUTHOR: Demirtunc et al. TITLE: Effects of Doxazosin and Amlodipine on Mean Platelet Volume and Serum Serotonin Level in Patients with Metabolic Syn-
a) drome YEAR: 2007
b) ANALYTE: Serotonin METHODE: ELISA
c) JOURNAL: Clinical Drug Investigation | NUMBER: 27(6) | PAGES: 435-441
8) AUTHOR: Kopczak et al. TITLE: N-nitroso-melatonin releases nitric oxide in the presence of serotonin and its derivatives YEAR: 2007
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Journal of Pineal Research | NUMBER: 43(4) | PAGES: 343-350
9) AUTHOR: Oben et al. TITLE: The effect of Cissus quadrangularis (CQR-300) and a Cissus formulation (CORE) on obesity and obesity-induced oxidative
a) stress YEAR: 2007
b) ANALYTE: Serotonin METHODE: ELISA
c) JOURNAL: Lipids Health Disease | NUMBER: 6 (4)
10) AUTHOR: Kälvegren TITLE: The Role of Chlamydia pneumoniae-induced Platelet Activation in Cardiovascular Disease YEAR: 2007
a) ANALYTE: Serotonin METHODE: ELISA
11) AUTHOR: Fornaro et al. TITLE: Prenatal Exposure to Fluoxetine Induces Fetal Pulmonary Hypertension in the rat YEAR: 2007
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Am J Resp Crit Care Med
12) AUTHOR: Kälvegren TITLE: The Role of Chlamydia pneumoniae-induced Platelet Activation in Cardiovascular Disease YEAR: 2007
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Dissertation - Linkoping University Sweden
13) AUTHOR: Theoharides et al. TITLE: Compositions for protection against superficial vasodilator flush syndrome, and methods of use YEAR: 2007
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: free patents online
14) AUTHOR: Karatay et al TITLE: Serum Serotonin Level and its Association with Pain in FMF Patients YEAR: 2007
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: The Eurasian Journal of Medicine
15) AUTHOR: Ho-Tin-Noé et al. TITLE: Platelet Granule Secretion Continuously Prevents Intratumor Hemorrhage YEAR: 2008
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Cancer Research | NUMBER: 68 | PAGES: 6851-6858
16) AUTHOR: Kidd et al. TITLE: Luminal regulation of normal and neoplastic human EC cell serotonin release is mediated by bile salts, amines, tastants,
a) and olfactants YEAR: 2008
b) ANALYTE: Serotonin METHODE: ELISA
c) JOURNAL: Am J Physiol Gastrointest Liver Physiol | NUMBER: 295 | PAGES: G260-G272
17) AUTHOR: Papaliodis et al. TITLE: The flavonoid luteolin inhibits niacin-induced flush YEAR: 2008
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: British Journal of Pharmacology | NUMBER: 153(7) | PAGES: 1382-1387
18) AUTHOR: Kälvegren et al. TITLE: Correlation between rises in Chlamydia pneumoniae -specific antibodies, platelet activation and lipid peroxidation after
a) percutaneous coronary intervention YEAR: 2008
b) ANALYTE: Serotonin METHODE: ELISA
c) JOURNAL: European Journal of Clinical Microbiology & Infectious Diseases | NUMBER: 27)7) | PAGES: 503-511
19) AUTHOR: Zolkowska et al. TITLE: Chronic Fenfluramine Administration Increases Plasma Serotonin (5-Hydroxytryptamine) to Nontoxic Levels YEAR: 2008
a) ANALYTE: Serotonin METHODE: RIA
b) JOURNAL: Journal of Pharmacology and Experimental Therapeutics | NUMBER: 324(2) | PAGES: 791-797
20) AUTHOR: Ghosh et al. TITLE: Platelet serotonin level and its correlation with finger length relation YEAR: 2008
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Indian Journal of Psychiatry | NUMBER: 50(4) | PAGES: 253-256
21) AUTHOR: Gritters et al. TITLE: Platelet activation in clinical haemodialysis: LMWH as a major contributor to bio-incompatibility? YEAR: 2008
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Nephrology Dialysis Transplantation
22) AUTHOR: Siddique et al. TITLE: KRJ-I and BON Cell Lines: Defining an Appropriate Enterochromaffin Cell Neuroendocrine Tumor Model YEAR: 2009
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Neuroendocrinology
23) AUTHOR: Duerschmied et al. TITLE: Serotonin stimulates platelet receptor shedding by tumor necrosis factor-alpha-converting enzyme (ADAM17) YEAR: 2009
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Journal of Thrombosis and Haemostasis
24) AUTHOR: Morando et al. TITLE: Fluoxetine treatment affects nitrogen waste excretion and osmoregulation in a marine teleost fish YEAR: 2009
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Aquatic Toxicology
25) UTHOR: Overmyer et al. TITLE: Assessment of the toxicological interaction of sertraline with cholinesterase inhibiting insecticides in aquatic insects using
a) the black fly, Simulium vittatum IS-7 YEAR: 2009
b) ANALYTE: Serotonin Research METHODE: ELISA
c) JOURNAL: Enviromental Toxicology
26) AUTHOR: Matondo et al. TITLE: Deletion of the serotonin transporter in rats disturbs serotonin homeostasis without impairing liver regeneration YEAR: 2009
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: American Journal of Physiology- Gastrointestinal and Liver Physiology | NUMBER: 296 | PAGES: G963-G968
27) AUTHOR: Adams et al. TITLE: Serotonergic lesions of the dorsal hippocampus differentially modulate locomotor hyperactivity induced by drugs of abuse
a) in rats: implications for schizophrenia YEAR: 2009
b) ANALYTE: Serotonin METHODE: ELISA
c) JOURNAL: Psychopharmacology
28) AUTHOR: Modlin er al. TITLE: Principal Component Analysis, Hierarchical Clustering, and Decision Tree Assessment of Plasma mRNA and Hormone Levels as an Early Detection Strategy for Small Intestinal Neuroendocrine (Carcinoid) Tumors
a) YEAR: 2009
b) ANALYTE: Serotonin METHODE: ELISA
c) JOURNAL: Ann Surg Oncol | NUMBER: 16 | PAGES: 487-498
29) AUTHOR: Kanerva et al. TITLE: Expression of Antizyme Inhibitor 2 in Mast Cells and Role of Polyamines as Selective Regulators of Serotonin Secretion YEAR: 2009
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: PLoS one | NUMBER: 4 (8) | PAGES: e6858
30) AUTHOR: Scheideler TITLE: Einfluss von kurz-, mittel- und langfristiger Belastung auf physiologische Parameter bei Arbeitshunden YEAR: 2009
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Dissertation
31) AUTHOR: Scheideler TITLE: Einfluss von kurz-, mittel- und langfristiger Belastung auf physiologische Parameter bei Arbeitshunden YEAR: 2009
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Dissertation
32) AUTHOR: Modlin et al. TITLE: Principal Component Analysis, Hierarchical Clustering, and Decision Tree Assessment of Plasma mRNA and Hormone Levels as an Early Detection Strategy for Small Intestinal Neuroendocrine (Carcinoid) Tumors
a) YEAR: 2009
b) ANALYTE: Serotonin METHODE: ELISA
c) JOURNAL: Annals of Surgial oncology | NUMBER: 2(16) | PAGES: 487-498
33) AUTHOR: Topsakal et al. TITLE: The relation between serotonin levels and insufficient blood pressure decrease during night-time in hypertensive patients YEAR: 2009
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Blood Pressure | NUMBER: 18(6) | PAGES: 367-371
34) AUTHOR: Mödder et al. TITLE: Relation of Serum Serotonin Levels to Bone Density and Structural Parameters in Women YEAR: 2009
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Journal of Bone and Mineral Research | NUMBER: 25(2) | PAGES: 415-422
35) AUTHOR: Wipfli et al. TITLE: An examination of serotonin and psychological variables in the relationship between exercise and mental health YEAR: 2009
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Scandinavian Journal of Medicine & Science in Sports
36) AUTHOR: Seddik et al. TITLE: Dried Leaf Extract Protects against Lead-Induced Neurotoxicity in Wistar Rats YEAR: 2010
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Europ J Scientific Res | NUMBER: 42 (1) | PAGES: 139-151
37) AUTHOR: Leu et al. TITLE: GPCR Somatostatin Receptor Extracellular Loop 2 Is a Key Ectodomain for Making Subtype-Selective Antibodies With
a) Agonist-Like Activities in the Pancreatic Neuroendocrine Tumor BON Cell Line YEAR: 2010
b) ANALYTE: Serotonin Research METHODE: ELISA
c) JOURNAL: Pancreas
38) AUTHOR: Svejda et al. TITLE: The 5-HT2B receptor plays a key regulatory role in both neuroendocrine tumor cell proliferation and the modulation of the
a) fibroblast component of the neoplastic microenvironment YEAR: 2010
b) ANALYTE: Serotonin METHODE: ELISA
c) JOURNAL: Cancer | NUMBER: 116 (12) | PAGES: 2902-2912
39) AUTHOR: Yu et al. TITLE: Brain Perivascular Macrophages and the Sympathetic Response to Inflammation in Rats After Myocardial Infarction YEAR: 2010
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Hypertension | NUMBER: 55 | PAGES: 652-659
40) AUTHOR: Rosado et al. TITLE: Serotonin transporter activity in platelets and canine aggression YEAR: 2010
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: The Veterinary Journal | NUMBER: 186(1) | PAGES: 104-105
41) AUTHOR: Saroya et al. TITLE: Injection of Resperpine into Zebrafish, Prevents Fish to Fish Communication of Radiation-Induced Bystander Signals: Confir- mation in Vivo of A Role for Serotonin in the Mechanism
a) YEAR: 2010
b) ANALYTE: Serotonin METHODE: ELISA
c) JOURNAL: Dose-Response | NUMBER: 8(3) | PAGES: 317-330
42) AUTHOR: Weaver et al TITLE: Early Events in Vascular Injury in the Rat Induced by the Phosphodiesterase IV Inhibitor SCH 351591 YEAR: 2010
a) ANALYTE: Histamine / Serotonin METHODE: ELISA
b) JOURNAL: Toxicology Pathology | NUMBER: 38 | PAGES: 738-744
43) AUTHOR: Schraenen et al. TITLE: Placental lactogens induce serotonin biosynthesis in a subset of mouse beta cells during pregnancy YEAR: 2010
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Diabetologia | NUMBER: 53 | PAGES: 2589-2599
44) AUTHOR: Dulcy et al. TITLE: Olfactory bulb serotonin level modulates olfactory recognition in the neonate rat YEAR: 2010
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Indian Journal of Experimental Biology (IJEB) | NUMBER: 48(11) | PAGES: 1078-1082
45) AUTHOR: Ganesh et al. TITLE: Role of olfactory bulb serotonin in olfactory learning in the greater short-nosed fruit bat, Cynopterus sphinx YEAR: 2010
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Brain Research | NUMBER: 1352 | PAGES: 108-117
46) AUTHOR: Li et al. TITLE: Inhibition of Sclerostin by Monoclonal Antibody Increases Bone Formation, Bone Mass, and Bone Strength in Aged Male
a) Rats YEAR: 2010
b) ANALYTE: Serotonin METHODE: ELISA
c) JOURNAL: Journal of Bone and Mineral Research | NUMBER: 25(12) | PAGES: 2647-2656
47) AUTHOR: Wie et al. TITLE: Molecular Depletion of Descending Serotonin Unmasks Its Novel Facilitatory Role in the Development of Persistent Pain YEAR: 2010
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: The Journal of Neuroscience | NUMBER: 30(25) | PAGES: 8624-8636
48) AUTHOR: Wijnvoord et al. TITLE: Inter-strain differences of serotonergic inhibitory pain control in inbred mice YEAR: 2010
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Molecular Pain | NUMBER: 6(70)
49) AUTHOR: Garcia-Faroldi et al. TITLE: Polyamines Are Present in Mast Cell Secretory Granules and Are Important for Granule Homeostasis YEAR: 2010
a) ANALYTE: Histamine/Serotonin METHODE: ELISA
b) JOURNAL: PLoS One | NUMBER: 5(11)
50) AUTHOR: Grah et al. TITLE: Serotonin and Cortisol as Suicidogenic Factors in Patients with PTSD YEAR: 2010
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Collegium Antropologicum | NUMBER: 34(4) | PAGES: 1433-1439
51) AUTHOR: Schoorl et al. TITLE: Platelet depletion, platelet activation and coagulation during treatment with hemodialysis YEAR: 2011
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Scandinavian Journal of Clinical and Laboratory Investigation
52) AUTHOR: Kreiner et al. TITLE: Fibroblast-specific expression of AC6 enhances ß-adrenergic and prostacyclin signaling and blunts bleomycin-induced
a) pulmonary fibrosis YEAR: 2011
b) ANALYTE: Serotonin METHODE: ELISA
c) JOURNAL: Pain | NUMBER: 152(5) | PAGES: 1127-1132
53) AUTHOR: Miyagi et al. TITLE: The Development of UDP-Glucuronosyltransferases 1A1 and 1A6 in the Pediatric Liver YEAR: 2011
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Drug Metabolism & Disposition | NUMBER: 39(5) | PAGES: 912-919
54) AUTHOR: Svejda et al. TITLE: Limitations in Small Intestinal Neuroendocrine Tumor Therapy by mTor Kinase Inhibition Reflect Growth Factor-Mediated
a) PI3K Feedback Loop Activation via ERK1/2 and AKT YEAR: 2011
b) ANALYTE: Serotonin METHODE: ELISA
c) JOURNAL: Cancer
55) AUTHOR: Kreiner et al. TITLE: Elevated muscle interstitial levels of pain-inducing substances in symptomatic muscles in patients with polymyalgia rheu-
a) matica YEAR: 2011
b) ANALYTE: Serotonin METHODE: ELISA
c) JOURNAL: Pain | NUMBER: 152(5) | PAGES: 1127-1132
56) AUTHOR: Kaushal et al. TITLE: CM156, a high affinity sigma ligand, attenuates the stimulant and neurotoxic effects of methamphetamine in mice YEAR: 2011
a) ANALYTE: Dopamine/ Serotonin METHODE: ELISA
b) JOURNAL: Neuropharmacology | NUMBER: 61(5-6) | PAGES: 992-1000
57) AUTHOR: Schneider et al. TITLE: Nitric oxide induced regulation of renal organic cation transport after renal ischemia/ reperfusion injury YEAR: 2011
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: American Journal of Physiology
58) AUTHOR: Magrini et al. TITLE: Serotonin-Mediated Tuning of Human Helper T Cell Responsiveness to the Chemokine CXCL 12 YEAR: 2011
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: PLoS One
59) AUTHOR: Ugun-Klusek et al. TITLE: Reduced placental vascular reactivity to 5-hydroxytryptamine in pre-eclampsia and the status of 5HT 2A receptors YEAR: 2011
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Vascular Pharmacology
60) AUTHOR: Ciuclan et al. TITLE: A Novel Murine Model of Severe Pulmonary Arterial Hypertension YEAR: 2011
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: American Journal of Respiratory and Critical Care Medicine
61) AUTHOR: Park et al. TITLE: The influence of host stress on the gastrointestinal tract and the microbiota YEAR: 2011
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Dissertation
62) AUTHOR: Sanner et al. TITLE: Effects of Delayed Laboratory Processing on Platelet Serotonin Levels YEAR: 2011
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Biological Research for Nursing
63) AUTHOR: El-Ansary et al. TITLE: Relationship between chronic lead toxicity and plasma neurotransmitters in autistic patients from Saudi Arabia YEAR: 2011
a) ANALYTE: Dopamine / Serotonin METHODE: ELISA
b) JOURNAL: Clinical Biochemistry | NUMBER: 44(23) | PAGES: 1116-1120
64) AUTHOR: Kannan et al. TITLE: Decreased cortical serotonin in neonatal rabbits exposed to endotoxin in utero YEAR: 2011
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Journal of Cerebral Blood Flow and Metabolism | NUMBER: 31 | PAGES: 738-749
65) AUTHOR: Mc Donald at el. TITLE: Higher levels of aggression are observed in socially dominant toadfish treated with the selective serotonin reuptake inhibi-
a) tor, fluoxetine YEAR: 2011
b) ANALYTE: Serotonin METHODE: ELISA
c) JOURNAL: Comparative Biochemistry and Physiology Part C | NUMBER: 153 | PAGES: 107-112
66) AUTHOR: Pichardo et al. TITLE: Increased serotonin concentration and tryptophan hydroxylase activity in reproductive organs of copulator males: a case of
a) adaptive plasticity YEAR: 2011
b) ANALYTE: Serotonin METHODE: ELISA
c) JOURNAL: Advances in Bioscience and Biotechnology | NUMBER: 2 | PAGES: 75-84
67) AUTHOR: Wixey et al. TITLE: Efficacy of post-insult minocycline administration to alter long-term hypoxia-ischemia-induced damage to the serotonergic
a) system in the immature rat brain YEAR: 2011
b) ANALYTE: Serotonin METHODE: ELISA
c) JOURNAL: Neuroscience | NUMBER: 182 | PAGES: 184-192
68) AUTHOR: Rajan et al. TITLE: Attenuation of 1-(m-Chlorophenyl)-Biguanide Induced Hippocampus-Dependent Memory Impairment by a Standardised
a) Extract of Bacopa monniera (BESEB CDRI-08) YEAR: 2011
b) ANALYTE: Dopamine / GABA / Serotonin METHODE: ELISA
c) JOURNAL: Neurochemical Research | NUMBER: 36(11) | PAGES: 2136-2144
69) AUTHOR: Malkesman et al. TITLE: Acute D-serine treatment produces antidepressant-like effects in rodents YEAR: 2011
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: The International Journal of Neuropsychopharmacology
70) AUTHOR: Koo et al. TITLE: Cochlear uptake of fluorescent gentamicin is increased in LPS-induced sepsis model YEAR: 2011
a) ANALYTE: Histamine / Serotonin METHODE: ELISA
b) JOURNAL: Dissertation
71) AUTHOR: Mostafa et al. TITLE: A lack of association between hyperserotonemia and the increased frecuency of serum anti-myelin basic protein auto-
a) antibodies in autistic children YEAR: 2011
b) ANALYTE: Serotonin METHODE: ELISA
c) JOURNAL: Journal of Neuroinflammation (JNI) | NUMBER: 8(71)
72) AUTHOR: Ciprandi et al. TITLE: Serotonin in Allergic Rhinitis: a Possible Role for Behavioural Symptoms YEAR: 2011
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Iran J Allergy Asthma Immunol | NUMBER: 10(3) | PAGES: 183-188
73) AUTHOR: Brainard et al. TITLE: Effects of Clopidogrel and Aspirin on Platelet Aggregation, Thromboxane Production, and Serotonin Secretion in Horses YEAR: 2011
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Journal of Veterinary Internal Medicine | NUMBER: 25 | PAGES: 116-122
74) AUTHOR: Mannaa et al. TITLE: Efficacy of fish liver oil and propolis as neuroprotective agents in pilocarpine epileptic rats treated with valproate YEAR: 2011
a) ANALYTE: Dopamine/ Serotonin METHODE: ELISA
b) JOURNAL: Pathophysiology | NUMBER: 18(4) | PAGES: 287-294
75) AUTHOR: Charles et al. TITLE: Bacopa monniera leaf extract up-regulates tryptophan hydroxylase (TPH2) and serotonin transporter (SERT) expression:
a) Implications in memory formation YEAR: 2011
b) ANALYTE: Serotonin METHODE: ELISA
c) JOURNAL: Journal of Ethnopharmacoloogy | NUMBER: 134 | PAGES: 55-61
76) AUTHOR: Wibowo et al. TITLE: Vitamin B? supplementation in pregnant women with nausea and vomiting YEAR: 2012
a) ANALYTE: Dopamine/ Serotonin METHODE: ELISA
b) JOURNAL: International Journal of Gynecology & Obstetrics | NUMBER: 116(3) | PAGES: 206-210
77) AUTHOR: Ohlsson et al. TITLE: Estrogen receptor-α expression in neuronal cells affects bone mass YEAR: 2012
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: PNAS
78) AUTHOR: Shahin et al. TITLE: Detection of Plasma and Urinary Monoamines and Their Metabolites in Nonsegmental Vitiligo YEAR: 2012
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Acta Dermatovenerol Croat | NUMBER: 20(1) | PAGES: 14-20
79) AUTHOR: Chin et al. TITLE: The role of mechanical forces and adenosine in the regulation of intestinal enterochromaffin cell serotonin secretion YEAR: 2012
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: American Journal of Physiology | NUMBER: 302(3) | PAGES: 397-405
80) AUTHOR: Siddiqi et al. TITLE: Identification of potential biomarkers of gold nanoparticle toxicity in rat brains YEAR: 2012
a) ANALYTE: Dopamine / Serotonin METHODE: ELISA
b) JOURNAL: Journal of Neuroinflammation (JNI) | NUMBER: 9(123)
81) AUTHOR: Sjögren et al. TITLE: The Gut Microbiota Regulates Bone Mass in Mice YEAR: 2012
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Journal of Bone and Mineral Research | NUMBER: 27(6) | PAGES: 1357-1367
82) AUTHOR: Brustad et al. TITLE: Structure-Guided Directed Evolution of Highly Selective P450-Based Magnetic Resonance Imaging Sensors for Dopamine
a) and Serotonin YEAR: 2012
b) ANALYTE: Serotonin METHODE: ELISA
c) JOURNAL: Journal of Molecular Biology
83) AUTHOR: Rokstad et al. TITLE: Microencapsulation of small intestinal neuroendocrine neoplasm cells for tumor model studies YEAR: 2012
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: The Official Journal of the Japanese Cancer Association | NUMBER: 103(7) | PAGES: 1230-1237
84) AUTHOR: Kaushal et al. TITLE: Pharmacological evaluation of SN79, a sigma (σ) receptor ligand, against methamphetamine-induced neurotoxicity in vivo YEAR: 2012
a) ANALYTE: Dopamine / Serotonin METHODE: ELISA
b) JOURNAL: European Neuropsychopharmacology
85) AUTHOR: Wang et al. TITLE: Correlation between serum serotonin and endoscopy inflammation scores in patients with ileal pouches YEAR: 2012
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Journal of Crohn?s and Colitis
86) AUTHOR: van den Buuse et al. TITLE: Exploring the Role of 5-HAT 1A Receptors in the Regulation of Prepulse Inhibition in Mice: Implications for Cross-Species
a) Comparisons YEAR: 2012
b) ANALYTE: Serotonin METHODE: ELISA
c) JOURNAL: ACS Chemical Neuroscience
87) AUTHOR: Baydar et al. TITLE: Evaluation of Tetrahydrobiopterin Pathway in Operating Room Workers: Changes in Biopterin Status and Tryptophan Meta-
a) bolism YEAR: 2012
b) ANALYTE: Serotonin METHODE: ELISA
c) JOURNAL: Bulletin of Environmental Contamination and Toxicology | NUMBER: 89(6) | PAGES: 1125-1128
88) AUTHOR: Srividhya et al. TITLE: Impact of epigallo catechin-3-gallate on acetylcholine-acetylcholine esterase cycle in aged rat brain YEAR: 2012
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Neurochemistry International | NUMBER: 60 | PAGES: 517-522
89) AUTHOR: Valiyaveettil et al. TITLE: Contribution of systemic factors in the pathophysiology of repeated blast-induced neurotrauma YEAR: 2013
a) ANALYTE: Serotonin METHODE: ELISA
b) JOURNAL: Neuroscience Letters | NUMBER: 539
90) AUTHOR: Santuzzi et al. TITLE: High-frequency transcutaneous electrical nerve stimulation reduces pain and cardio-respiratory parameters in an animal
a) model of acute pain: Participation of peripheral serotonin YEAR: 2013
b) ANALYTE: Serotonin METHODE: ELISA
c) JOURNAL: Physiotherapy Theory and Practice
91) AUTHOR: Damen et al. TITLE: The Stimulatory Adenosine Receptor ADORA2B Regulates Serotonin (5-HT) Synthesis and Release in Oxygen-Depleted EC
a) Cells in Inflammatory Bowel Disease YEAR: 2013
b) ANALYTE: Serotonin METHODE: ELISA
c) JOURNAL: Plos One | NUMBER: 8(4)
92) AUTHOR: Tang et al. TITLE: Back propagation artificial neural network for community Alzheimer´s disease screening in China YEAR: 2013
a) ANALYTE: Dopamine/ Serotonin METHODE: ELISA
b) JOURNAL: Neutral Regeneration Research | NUMBER: 8(3) | PAGES: 270-276
10. LDN 公司和其他产品介绍：
德国LDN，全名labor diagnostika nord，是生物胺、神经递质检测的行业领先公司。 本试剂盒可用于人/大鼠/小鼠/各种动物样本，血清/抗凝血浆样本。
LABOR DIAGNOSTIKA NORD在德国始建于1996年， 目的是向临床和科研领域产品提供优质的检测产品和系统。最早的生物胺检测ELISA试剂，是LDN的ELISA系列核心，目前是全世界最大的生物胺检测试剂提供商；推荐特色产品是儿茶酚胺（catecholamines）检测系列，Histamine（组胺）检测ELISA，5-羟色胺（血清素）ELISA 试剂盒，自由Metanephrine（3-甲氧基肾上腺素）在血浆中的检测ELISA ；
随后发展的内分泌检测系列和食物安全系列为德国LDN 另外两大产品系列。
LABOR DIAGNOSTIKA NORD 产品在全世界八十多个国家通过代理商或者OEM 合作伙伴进行销售，在北美洲通过子公司Rocky Mountain Diagnostics（ROCKY mountain诊断公司）进行销售。
优势产品有：Histamine（组胺） ELISA ，Noradrenaline（去甲肾上腺素） ELISA, Dopamine（多巴胺）ELISA, Serotonin ( 血清素，又名5羟色胺）ELISA等。根据不同的样本类型，分为Fast Track （快速），research 型，高敏型等多种类型；既有针对所有类型样本的research类型的，也有针对血清，血浆或者尿液的试剂盒；这些试剂盒都可用于所有animal的检测（如人，小鼠，大鼠，其他动物模型等）。
具体见下表：
德国LDN生物胺，神经递质ELSIA 试剂盒:
指标主要有：儿茶酚胺（catecholamines）检测试剂盒：Adrenaline（肾上腺素），Dopamine （多巴胺），Noradrenaline（去甲肾上腺素）；
以及组合检测试剂盒：2-CAT（A-N）， 2-CAT（N-D），3-CAT；
Histamine（组胺），有针对尿液，血清，全血检测的试剂盒，也有针对细胞上清/尿液/全血检测的试剂盒；还有针对所有样本检测的试剂盒；检测粪便的组胺试剂盒；
Metanephrine（3-甲氧基肾上腺素），Normetanephrine（去甲-3-0-甲基肾上腺素），Serotonin（5-HT，色氨酸，5羟色胺），5-HIAA（5-吲哚乙酸），Glutamate（谷氨酸），GABA（氨基丁酸），Tryptophan（色氨酸）；
既有针对所有类型样本的research类型的，也有针对血清，血浆或者尿液的试剂盒；
这些试剂盒都可用于所有animal的检测（如人，小鼠，大鼠，牛，羊，猪，豚鼠，猴，兔等其他动物模型）。