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德国ChromoTek公司推出一系列蛋白与抗体产品
ChromoTek公司蛋白与抗体产品
德国ChromoTek公司成立于2008年,致力于细胞生物学和蛋白质组学的研发与研究,公司拥有强大的研发团队,有先进的生物制剂,主要主要使命是花费较少的时间和成本,使客户获得得高质量的实验数据,
Nano-Traps基于alpaca的单域抗体片段具有分子量小(15Kda)、极端稳定(70℃仍保持稳定)高亲和力(高特异性性结合GFP蛋白)、孵化时间短(只需5-30min)无普通抗体轻链与重链污染等一系列优点,能够更好与agarose beads或者magnetic beads结合,从细胞提取物或细胞器中定量分离GFP融合蛋白及结合因子,在免疫沉淀或蛋白质相互作用的实验中,可高效抓取或纯化GFP或RPF融合蛋白,被广泛用于免疫沉淀、质谱分析、酶活测定、生物大分子相互作用/亲和力实验、染色质免疫沉淀(ChIP)、蛋白质相互作用分析。
Nano-Traps主要产品见表:
|
品牌 |
货号 |
品名 |
规格 |
价格 |
GFP-Trap® |
Chromo tek |
gta-10 |
GFP-Trap®, coupled to agarose |
10 rxns |
2000 |
Chromo tek |
gta-20 |
GFP-Trap®, coupled to agarose |
20 rxns |
6700 |
Chromo tek |
gta-100 |
GFP-Trap®, coupled to agarose |
100 rxns |
27090 |
Chromo tek |
gta-200 |
GFP-Trap® coupled to agarose |
200 rxns |
48240 |
Chromo tek |
gta-400 |
GFP-Trap® coupled to agarose |
400 rxns |
84330 |
Chromo tek |
gtak-20 |
GFP-Trap® coupled to agarose kit |
20 rxns |
8400 |
Chromo tek |
gtm-20 |
GFP-Trap® coupled to magnetic beads |
20 rxns |
6700 |
Chromo tek |
gtm-100 |
GFP-Trap® coupled to magnetic beads |
100 rxns |
27090 |
Chromo tek |
gtm-200 |
GFP-Trap® coupled to magnetic beads |
200 rxns |
5360 |
Chromo tek |
gtp-96 |
GFP-multiTrap®, black 96 well plate |
1 pc |
4100 |
Chromo tek |
gtp-96 |
GFP-multiTrap®, black 96 well plate |
5 pc |
15600 |
Chromo tek |
gtm-400 |
GFP-Trap® coupled to magnetic beads |
400 rxns |
84330 |
Chromo tek |
gtmk-20 |
GFP-Trap® coupled to magnetic beads kit |
20 rxns |
8400 |
Chromo tek |
gt-250 |
GFP-Trap® free protein |
250 µg |
4500 |
RFP-Trap® |
Chromo tek |
rta 10 |
RFP-Trap®, coupled to agarose |
10rxns |
2000 |
Chromo tek |
rta 20 |
RFP-Trap®, coupled to agarose |
20rxns |
6700 |
Chromo tek |
rta 100 |
RFP-Trap®, coupled to agarose |
100 rxns |
27090 |
Chromo tek |
rta200 |
RFP-Trap®, coupled to agarose |
200rxns |
5360 |
Chromo tek |
rta 400 |
RFP-Trap®, coupled to agarose |
400rxns |
84330 |
Chromo tek |
rtak-20 |
RFP-Trap®, coupled to agarose, |
20 rxns |
8400 |
Chromo tek |
rt-250 |
RFP-Trap®, free protein |
250 µg |
4500 |
GST-Trap |
Chromo tek |
stm-10 |
GST-Trap®, coupled to magnetic beads |
10 rxns |
2000 |
Chromo tek |
stm-20 |
GST-Trap®, coupled to magnetic beads |
20 rxns |
6700 |
Chromo tek |
stm-100 |
GST-Trap®, coupled to magnetic beads |
100 rxns |
27090 |
Chromo tek |
stm-200 |
GST-Trap®, coupled to magnetic beads |
200 rxns |
48240 |
Chromo tek |
stm-400 |
GST-Trap®, coupled to magnetic beads |
400 rxns |
84330 |
Chromo tek |
stmk-20 |
GST-Trap®, coupled to magnetic beads, kit |
20 rxns |
8400 |
Chromo tek |
st-250 |
GST-Trap®, free protein |
250 µg |
4500 |
Dnmt1-Trap |
Chromo tek |
dta-10 |
Dnmt1-Trap, coupled to agarose |
10 rxns |
2000 |
Chromo tek |
dta-20 |
Dnmt1-Trap, coupled to agarose |
20 rxns |
6700 |
Chromo tek |
dta-100 |
Dnmt1-Trap, coupled to agarose |
100 rxns |
27090 |
Chromo tek |
dta-200 |
Dnmt1-Trap, coupled to agarose |
200 rxns |
48240 |
Chromo tek |
dta-400 |
Dnmt1-Trap, coupled to agarose |
400 rxns |
84330 |
Chromo tek |
dtak-20 |
Dnmt1-Trap, coupled to agarose, kit |
20rxns |
8400 |
Chromo tek |
dt-250 |
Dnmt1-Trap,freeprotein |
250µg |
4500 |
Binding Controls |
Chromo tek |
bab-20 |
Blocked agarose beads (binding control) |
500 µl (20 rxns) |
1500 |
Chromo tek |
bmp-20 |
Blocked magnetic particles ( binding control) |
500 µ l ( 20 rxns) |
1500 |
Nano-booster是利用Atto公司的优质荧光素共价结合到GFP-booster上的一种荧光增强剂,对变性荧光蛋白具有再激活作用,不仅能够增强信号,还能够增加荧光蛋白荧光稳定性。GFP-booster、RFP-booster是一种小分子、高特异性的GFP结合蛋白,可以高效、高特异性的结合GFP。在HCL处理的BrdU、EdU-Click-iT™、FISH等实验中,热变性处理会干扰荧光融合蛋白信号的检测,Nano-booster主要用于这种生理表达水平下细胞样本中荧光融合蛋白表达的信号强度弱、荧光融合蛋白的耐光性和量子效率一般达不到超分辨率显微镜要求等生物学实验。
Nano-booster主要产品见下表:
|
|
货号 |
品名 |
规格 |
价格 |
GFP-Booster |
Chromo tek |
gba488-TS |
GFP-Booster_atto488-TS |
50 µg |
2500 |
Chromo tek |
gba488 |
GFP-Booster_atto488 |
100 µg |
5400 |
Chromo tek |
gba-647N |
GFP-Booster-Atto 647N |
100 µg |
询价 |
Chromo tek |
gbdl-650-TS |
GFP-Booster- Atto 647N |
50 µg |
询价 |
Chromo tek |
gba-594 |
GFP-Booster-Atto 594 |
100 µg |
询价 |
RFP-Booster |
Chromo tek |
rba594-TS |
RFP Booster atto594 TS |
50u g |
2500 |
Chromo tek |
rba594 |
RFP-Booster_atto594 |
100 µg |
5400 |
Chromo tek |
rbdl-650-TS |
RFP-Booster- Atto 650ts |
50 μg |
询价 |
Chromo tek |
rba-700 |
RFP-Booster-Atto 700 |
100 μg |
询价 |
Chromo tek |
rba-488 |
RFP-Booster-Atto 488 |
100 μg |
询价 |
ChromoTek推出一系列高质量常见抗体见下表:
货号 |
品牌 |
品名 |
规格 |
价格 |
2e8 |
Chromo tek |
Dnmt1antibody,ratmonoclonal |
500µl |
4900 |
2e8ts |
Chromo tek |
Dnmt1antibody,ratmonoclonal |
125µl |
2000 |
6g9 |
Chromo tek |
GST antibody, rat monoclonal |
100 µ l |
3900 |
3f5 |
Chromo tek |
RFP antibody mouse monoclonal |
100 µg |
3900 |
5f8 |
Chromo tek |
RFP antibody rat monoclonal |
100 µg |
3900 |
3h9 |
Chromo tek |
GFP antibody, rat monoclonal |
100 µg |
3900 |
16d10 |
Chromo tek |
PCNA antibody rat monoclonal |
100 µg |
4900 |
16d10ts |
Chromo tek |
PCNA antibody rat monoclonal |
25 µg |
2000 |
3h9 |
Chromo tek |
GFP antibody, rat monoclonal |
100 µg |
3900 |
9.00E+01 |
Chromo tek |
c-Myc antibody, rat monoclonal |
100 µg |
3900 |
6f7 |
Chromo tek |
FLAG antibody, rat monoclonal |
100 µg |
3900 |
7c9 |
Chromo tek |
HA antibody, rat monoclonal |
100 µg |
3900 |
4b8 |
Chromo tek |
Ago1 antibody, rat monoclonal |
100 µg |
5400 |
4b8ts |
Chromo tek |
Ago1 antibody, rat monoclonal |
25 µg |
2000 |
11a9 |
Chromo tek |
Ago2 antibody, rat monoclonal |
100 µg |
5400 |
11a9ts |
Chromo tek |
Ago2 antibody, rat monoclonal |
25 µg |
2000 |
1c7 |
Chromo tek |
RNA Pol II CTD, unphosphorylated CTD |
5 ml |
5400 |
1c7ts |
Chromo tek |
RNA Pol II CTD, unphosphorylated CTD |
1 ml |
2000 |
3d12 |
Chromo tek |
RNA Pol II CTD, phos. Tyrosine 1 |
5 ml |
5400 |
3d12ts |
Chromo tek |
RNA Pol II CTD, phos. Tyrosine 1 |
1 ml |
2000 |
3e10 |
Chromo tek |
RNA Pol II CTD, phos. Serine 2 |
5 ml |
5400 |
3e10ts |
Chromo tek |
RNA Pol II CTD, phos. Serine 2 |
1 ml |
2000 |
6d7 |
Chromo tek |
RNA Pol II CTD, phos. Threonine 4 |
5 ml |
5400 |
6d7ts |
Chromo tek |
RNA Pol II CTD, phos. Threonine 4 |
1 ml |
2000 |
3e8 |
Chromo tek |
RNA Pol II CTD, phos. Serine 5 |
5 ml |
5400 |
3e8ts |
Chromo tek |
RNA Pol II CTD, phos. Serine 5 |
1 ml |
2000 |
4e12 |
Chromo tek |
RNA Pol II CTD, phos. Serine 7 |
5 ml |
5400 |
4e12ts |
Chromo tek |
RNA Pol II CTD, phos. Serine 7 |
1 ml |
2000 |
Chromobodies是一种极其微小的细胞功能抗体,它是基于融合有荧光蛋白的来自羊驼的抗体重链的抗原结合域结构。与常规的抗体相比,这些新的荧光纳米探针可以实现活细胞结构和进程的实时分析和可视化,是研究细胞HCA分析的一种新的理想试剂。Chromobodies除了能够得到稳定的细胞株,还可以得到Chromobodies质粒,不会干扰内源性蛋白的功能,能够更好的标记活细胞中的天然蛋白。
Chromobodies系列产片见下表:
货号 |
Chromo tek |
品名 |
规格 |
价格 |
dcg |
Chromo tek |
Dnmt1-Chromobody®plasmid,αDnmt1-GFP |
20µg |
14900 |
dcr |
Chromo tek |
Dnmt1-Chromobody®plasmid,αDnmt1-RFP |
20µg |
14900 |
ccg |
Chromo tek |
Cell Cycle Chromobody® plasmid αPCNA GFP |
20 µg |
14900 |
ccr |
Chromo tek |
Cell Cycle Chromobody® plasmid PCNA RFP |
20 µg |
14900 |
lcg |
Chromo tek |
Lamin Chromobody® plasmid, αLamin-GFP |
20 µg |
14900 |
lcr |
Chromo tek |
Lamin Chromobody® plasmid, αLamin-RFP |
20 µg |
14900 |
acg |
Chromo tek |
Actin Chromobody® plasmid, αActin-GFP |
20 µg |
14900 |
acr |
Chromo tek |
Actin Chromobody® plasmid, αActin-RFP |
20 µg |
14900 |
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