RT-QPCR Primer Assays are designed for SYBR Green based on real-time PCR detection. The primer design computer algorithm has been developed using an in vitro assay to ensure that the resulting primer sequences generate a single PCR product of the predicted size and a minimal amount of primer–dimer in 30 cycles of PCR amplification. The assay also ensures that the amplification efficiency of the primers is at least 90%. As a result, the algorithm designs highly effective primer sequences for SYBR Green based real-time PCR detection. RT-QPCR Primer Assays are available for every human, mouse, rat, rhesus macaque, fruit fly, and dog gene annotated by the NCBI.

Uniform Reaction Condition: Reduce experimental design time

High Specificity: Absence of non-specific amplification and primer-dimers ensures reproducible data

Validation and Precision:
a) Human-,mouse-,rat-and rabbit specific primers are designed by a proprietary algorithm and validated for precision performance.
b) Primer validation includes melting curve to ensure amplification of the correct target DNA.

*Store Nuclease-free Water at -20℃、4℃ or room temp.

qPCR Detection Kit is shipped at ambient temperature, But for long time storage, RT-QPCR Detection kit can be used within 12 mouths if the whole kit is stored at -20℃

Note: We suggest adjusting RNA concentration so that 1 μL is used

Attention: Roche LightCycler 480® Users: Adjust the ramp rate to 1ºC/sec.

1. The 10-minute step at 95ºC is required to activate the HotStart DNA polymerase.

2. Detect and record SYBR Green fluorescence from every well during the annealing step of each cycle.

3. Different instruments need different lengths of time to detect the fluorescent signal. Choose the appropriate time for the annealing step (55ºC) for your instrument.

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